Resources for your Immunological Research, from detecting HLA-antibody interactions to analysis of immune cells
HLA Class II Proteins
Pure Protein’s technologically modified HLA-Class II proteins are available in soluble form at high purity. Select from a wide range of functionally intact and zipper stabilized alleles to identify, profile, or monitor antigen-specific immune responses from antibodies to immune cells.
HLA Class II Proteins
Resources for your Immunological Research, from detecting HLA-antibody interactions to analysis of immune cells
Pure Protein’s technologically modified HLA-Class II proteins are available in soluble form at high purity. Select from a wide range of functionally intact and zipper stabilized alleles to identify, profile, or monitor antigen-specific immune responses from antibodies to immune cells.
Choose from a range of HLA alleles
HLA-DQ
Select from more than 60 HLA-DQ alleles to detect and monitor humoral or cellular antigen-specific immune responses
HLA-DP
Choose from a pool of over 50 HLA-DP alleles to research the variety of heterodimeric isoforms
HLA-DR
Discover a selection of over 40 HLA-DR alleles, the emerging marker for histocompatibility screening
HLA Class II Basics
Soluble HLA (sHLA) molecules are recombinant and naturally folded glycoproteins. Using proprietary technology, Pure Protein truncates the two protein subunits just before the trans-membrane and cytoplasmic domain and stabilizes them by inserting a leucine zipper (ZP) that allows easy secretion and purification without associated cell membrane portions.
Structurally, soluble HLA class II proteins are heterodimeric glycoproteins consisting of an alpha chain (comprised of α1, α2 domains), a beta chain (comprised of β1, β2 domains) and an endogenously loaded peptide they present forming a trimeric complex.
Protein Characteristics
HLA Class II genes are highly polymorphic, encoding thousands of different alleles and potentially recombine to create isoform by combination of alpha and beta chains. Pure Protein offers a broad selection of single-specificity HLA proteins covering not only population frequency but also isoform availability. Like their Class I counterpart, sHLA Class II molecules also lack their transmembrane portions and are readily secreted allowing economic production and purification eliminating detergent cell lysates interferences.
Soluble Class II proteins are well characterized, proven to present native, antigenic epitopes recognized by specific antibodies, demonstrate high stability and integrity of their structures and are most compliable to most application platforms as well as highly adaptable to individual needs.
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Soluble HLA Class I Proteins
Mammalian produced, endogenously loaded, and naturally folded recombinant soluble HLA Class I proteins
Soluble HLA Class II Proteins
Mammalian produced, zipper stabilized, endogenously loaded, and naturally folded recombinant soluble HLA Class II proteins
Peptide Validation and Screening Services
Competition-based HLA peptide binding assays for the validation and screening of putative T cell epitopes
