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Peptide Validation and Screening Services

To design improved vaccination and therapeutic targeting strategies, it is critical to identify and validate epitopes that can be recognized and targeted by the immune system to prevent or treat various types of disease conditions.

Overcome the challenge of selecting the "right" targets with a suite of assays aimed at providing critical insight into epitope interactions. Our T-cell peptide epitope discovery and validation services provide solutions and essential information to advance qualified candidates quickly through the development process.

Choose from a range of services

Peptide Epitope Validation

Validate individual peptide epitope candidates by determining their physical binding characteristics and directly measure accurate IC50 values in order to judge and properly prioritize their immunogenic potential.

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Peptide Epitope Screening

Screen focused peptide libraries with the goal of identifying the highest ranking, potentially immunogenic epitopes while simultaneously eliminating non-binders to accelerate your discovery process and to save valuable resources and costs.

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T cell Epitope Mapping

Discover your next immunotherapy targets utilizing a high-throughput approach to create individual epitope maps on any chosen protein that is potentially recognized by T cells.

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Peptide Epitope Validation

Competition based peptide binding assay methodologies have become exceedingly popular for assessing the ability of synthetically defined peptide epitopes to associate with specific HLA complexes. This has been accomplished by determining their half maximal inhibitory concentration (IC50) as a measure of the effectiveness of inhibiting test peptides to judge their immunogenic potential and value in the development of novel immunotherapies.

Advance qualified peptide candidates quickly by validating your screening candidates or predicted epitopes by confirming their affinity and ranking order.

Fluoresence polarization-based competition assay to determine the IC50 of soluble HLA to specific peptides
Screening results of HLA peptide affinity

Peptide Epitope Screening

The peptide epitope screening system interrogates peptide libraries with variable sequence data. Pure Protein’s screening system identifies epitope candidates and properly prioritizes these newly found potential T-cell epitopes according to their HLA affinity. Peptide epitope screening efforts are primarily applied to eliminate false positive and negative results generated by computer algorithms. The screening system also resolves restriction elements and allows researchers to compare variable and conserved regions of different clades of a virus.

T Cell Epitope Mapping

T cell epitope mapping has emerged as one of the most powerful new drug discovery tools for a range of biomedical applications. Our mapping service is a high-throughput approach, which allows the creation of individual epitope maps for any protein of interest. This application enables the screening of large libraries of overlapping peptides with a broad selection of HLA alleles to identify high affinity binding peptides and the selection of the most potent T cell epitope candidates.

Epitope mapping of influenza peptides

Fluorescence Polarization Technology

Fluorescence polarization (FP) is unique among methods used to analyze molecular binding events because it allows the instantaneous measurement of the ratio between free and bound labeled ligand in solution without any separation steps. The technology is based on the principle that if a fluorescent-labeled peptide binds to a sHLA molecule of higher molecular weight, polarization values will increase due to the slower molecular rotation of the bound probe.

Competitive Assay Technology

To elaborate the fast and precise molecular binding of a peptide to an HLA molecule, a reference fluorescent-labeled peptide is incubated with activated sHLA in the presence of a peptide competitor and peptide/HLA interaction is monitored over time. Only a simple three step process is required to perform the assay. The activation step (1) is forcing the folded structure into a temporary state of instability, making it amenable for the competitor peptide to be inserted into the groove. The mixing step (2) is providing a fluorescent-labeled tracer peptide (yellow) and test peptide (blue) for competition. And lastly, the measuring step (3) where data is collected, and a positive response will occur when the peptide of interest outcompetes the labeled peptide tracer. A negative response will take place when the peptide of interest has no binding characteristics and only the tracer is assembling with the sHLA.

Fluorsence polarization binding assay
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Advantages

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Customized Selection

Choose from a wide range of major HLA alleles stably expressed in mammalian cell lines and available in large quantities
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Speed

Profit from short turnaround times with results available in as little as 3 days upon receipt of the peptides
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No Radioisotopes

Our peptide binding assays do not use radioisotopes and are suitable for high-throughput applications
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State of the Art Detection System

All assays are based on Fluorescence Polarization, allowing direct measurement of the ratio between bound and free in real-time
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Highly Accurate

Fluorescence polarization-based assays have high accuracy and reproducibility with data outputs easy to interpret
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Homogeneous Assays

HLA peptide binding assays are homogeneous in nature with no antibody involvement, cumbersome ligand/complex separation or washing steps

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